Before Start

Important Note

What is YAP?

YAP (Yet Another-Pipeline ) is a mapping pipeline designed for several single nucleus methyl-cytosine sequencing (snmC-seq) based technologies (see below).

It starts from demultiplexing FASTQ files and ends at generating single-cell ALLC files.

See source code on Github.

Technologies Supported

How to use it?

  1. Be familiar with the background of cell barcoding and the technology you use. If not, read the corresponding TECH BACKGROUND section.

  2. Follow the installation tutorial to install yap and associated packages.

  3. Follow the MAPPING PIPELINE section step by step.

  4. If you have questions, first go to the FAQ page. If the problem is not solved, you can post an issue on GitHub.

  5. This documentation is searchable.

After Mapping?

Check out ALLCools, the package for downstream cell-level and cluster-level analysis.

Authors

Hanqing Liu

Jingtian Zhou

Chongyuan Luo

Wei Tian

Last updated

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