In V1 barcoding, we have 8 random indexes to barcode two 384-well plates (768 wells/cells) together. This results in a 96-Well plate, which has 4 wells/cells' DNA been merged together. We then use a quarter (96) of the PCR indexes (we have 384 kinds of PCR indexes in total, so 96 is a quarter) to barcoding each well of the 96-well plate. Then we can merge them together. For a typical eight-plate experiment (3072 wells/cells in total), we will use 8 * 96 * 4 barcode combinations.